2025

  • Dho, Y.; Smith, K.; Sattely, E. S. “Discovery of homoharringtonine pathway enzymes reveals a whole plant model for coordinated biosynthesis” bioRxiv 2025 doi: https://doi.org/10.1101/2025.08.26.672243

  • Lowensohn, T. N.*; Cody, W. B.*; Tsai, C.; Vlahos, A.E.; Call, C. C.; Gao, X. J., Sattely, E. S. “An in planta single-cell screen to accelerate functional genetics” bioRxiv 2025 doi: https://doi.org/10.1101/2025.08.06.668818

  • McClune, C. J.*; Liu, J. C.-T.*; Wick, C.; De La Peña, R.; Lange, B. M.; Fordyce, P. M.; Sattely, E. S. “Discovery of FoTO1 and Taxol genes enables biosynthesis of baccatin III” Nature 2025 doi: https://doi.org/10.1038/s41586-025-09090-z

    Highlighted in Science, C&EN, and Nature

2024

  • Niehs, S. P.; Rajniak, J.; Johnson, A.; Wengier, D. L.; Sattely, E. S. “Zinc-Starved Brassicaceae Plants Secrete Peptides That Induce Root Expansion” bioRxiv 2024 doi: https://doi.org/10.1101/2024.06.11.598559

  • Fischer, C.; Jeon, J. E.; Smith, K. B.; Sattely, E. “Specific Derivatization of Internal Alkynes for Improved Electrospray Analysis” ChemRxiv 2024 doi: https://doi.org/10.26434/chemrxiv-2024-kn67s

  • Blum, J. E.; Kong, R.; Schulman, E. A.; Chen, F. M.; Upadhyay, R.; Romero-Meza, G.; Littman, D. R.; Fischbach, M. A.; Nagashima, K. & Sattely, E. S. “Discovery and Characterization of Dietary Antigens in Oral Tolerance” bioRxiv 2024 doi: https://doi.org/10.1101/2024.05.26.593976

  • Liou, C. S.; Iakiviak, M; Rajniak, J.; Murugkar, P. P.; Higginbottom, S. K.; Jasper, M.; Fischbach, M. A.; Sattely, E. S. “Gut microbiota gate host exposure to metabolites from dietary Solanums” bioRxiv 2024 doi: https://doi.org/10.1101/2024.03.20.584512

  • Foret, J.; Kim, J.-G.; Sattely, E. S.; Mudgett, M. B. “Transcriptome Analysis Reveals Role for WRKY70 in EarlyN-Hydroxy-Pipecolic Acid Signaling” bioRxiv 2024 doi: https://doi.org/10.1101/2024.04.23.590810

  • Liu, J. C.-T.; De La Peña, R.; Tocol, C.; Sattely, E. S. “Reconstitution of Early Paclitaxel Biosynthetic Network” Nat. Commun. 2024 doi: https://doi.org/10.1038/s41467-024-45574-8

2023

  • Nett, R. S.; Dho, Y.; Tsai, C.; Passow, D.; Martinez Grundman, J.; Low, Y.-Y.; Sattely, E. S. “Plant Carbonic Anhydrase-like Enzymes in Neuroactive Alkaloid Biosynthesis” Nature 2023 doi: https://doi.org/10.1038/s41586-023-06716-y

  • Nagashima, K.; Zhao, A.; Atabakhsh, K.; Bae, M.; Blum, J. E.; Weakley, A.; Jain, S.; Meng, X.; Cheng, A. G.; Wang, M.; Higginbottom, S.; Dimas, A.; Murugkar, P.; Sattely, E. S.; Moon, J. J.; Balskus, E. P.; Fischbach, M. A. “Mapping the T Cell Repertoire to a Complex Gut Bacterial Community” Nature 2023 doi: https://doi.org/10.1038/s41586-023-06431-8

  • Carlson, E. D.; Rajniak, J.; Sattely, E. S. “Multiplicity of the Agrobacterium Infection of Nicotiana Benthamiana for Transient DNA Delivery” ACS Synth. Biol. 2023 doi: https://doi.org/10.1021/acssynbio.3c00148

  • Mehta, N.; Meng, Y.; Zare, R.; Kamenetsky-Goldstein, R.; Sattely, E. S “Developmental Gradient Reveals Biosynthetic Pathways to Eukaryotic Toxins in Monocot Geophytes” bioRxiv 2023 doi: https://doi.org/10.1101/2023.05.12.540595

  • Kaseniit, K. E.; Katz, N.; Kolber, N. S.; Call, C. C.; Wengier, D. L.; Cody, W. B.; Sattely, E. S.; Gao, X. J. Author Correction: “Modular, Programmable RNA Sensing Using ADAR Editing in Living Cells” Nat. Biotechnol. 2023 doi: https://doi.org/10.1038/s41587-022-01617-3

  • Kaseniit, K. E.; Katz, N.; Kolber, N. S.; Call, C. C.; Wengier, D. L.; Cody, W. B.; Sattely, E. S.; Gao, X. J. Modular, “Programmable RNA Sensing Using ADAR Editing in Living Cells” Nat. Biotechnol. 2023 doi: https://doi.org/10.1038/s41587-022-01493-x

  • De La Peña, R.* & Hodgson, H.* & Liu, J. C. T.*; Stephenson, M. J.; Martin, A. C.; Owen, C.; Harkess, A.; Leebens-Mack, J.; Jimenez, L. E.; Osbourn, A. & Sattely, E. S. “Complex scaffold remodeling in plant triterpene biosynthesis” Science 2023 doi: https://doi.org/10.1126/science.adf1017

    Highlighted in C&EN

  • Khariton, M.; McClune, C. J.; Brower, K. K.; Klemm, S.; Sattely, E. S.; Fordyce, P. M. & Wang, B. “Alleviating Cell Lysate-Induced Inhibition to Enable RT-PCR from Single Cells in Picoliter-Volume Double Emulsion Droplets” Anal. Chem. 2023 doi: https://doi.org/10.1021/acs.analchem.2c03475

2022

  • Kim, S. S.; Wengier, D. L.; Ragland, C. J.; Sattely, E. S. “Transcriptional Reactivation of Lignin Biosynthesis for the Heterologous Production of Etoposide Aglycone in Nicotiana benthamiana” ACS Synth. Biol. 2022 doi: https://doi.org/10.1021/acssynbio.2c00289

2021

  • Khlystov, N. A.; Yoshikuni, Y.; Deutsch, S.; Sattely, E. S. “A Plant Host, Nicotiana benthamiana, Enables the Production and Study of Fungal Lignin-Degrading Enzymes” Commun. Biol. 2021 doi: https://doi.org/10.1038/s42003-021-02464-9

  • Schnabel, T.; Sattely, E. “Engineering Posttranslational Regulation of Glutamine Synthetase for Controllable Ammonia Production in the Plant Symbiont Azospirillum brasilense” Appl. Environ. Microbiol. 2021 doi: https://doi.org/10.1128/AEM.00582-21

  • Nett, R. S.; Dho, Y.; Low, Y.-Y.; Sattely, E. S. “A Metabolic Regulon Reveals Early and Late Acting Enzymes in Neuroactive Lycopodium Alkaloid Biosynthesis” Proc. Natl. Acad. Sci. U. S. A. 2021 doi: https://doi.org/10.1073/pnas.2102949118

  • Holmes, E. C.; Chen, Y.-C.; Mudgett, M. B. & Sattely, E. S. “Arabidopsis UGT76B1 Glycosylates N-Hydroxy-Pipecolic Acid and Inactivates Systemic Acquired Resistance in Tomato” Plant Cell 2021 doi: https://doi.org/10.1093/plcell/koaa052

  • Kim, S. S.; Sattely, E. S. “Dirigent Proteins Guide Asymmetric Heterocoupling for the Synthesis of Complex Natural Product Analogues” J. Am. Chem. Soc. 2021 doi: https://doi.org/10.1021/jacs.0c13164

2020

  • Harbort, C. J.* & Hashimoto, M*.; Inoue, H.; Niu, Y.; Guan, R.; Rombolà, A. D.; Kopriva, S.; Voges, M. J. E. E. E.; Sattely, E. S.; Garrido-Oter, R. & Schulze-Lefert, P. “Root-Secreted Coumarins and the Microbiota Interact to Improve Iron Nutrition in Arabidopsis” Cell Host Microbe 2020 doi: https://doi.org/10.1016/j.chom.2020.09.006

  • Calgaro-Kozina, A.; Vuu, K. M.; Keasling, J. D.; Loqué, D.; Sattely, E. S. & Shih, P. M. “Engineering Plant Synthetic Pathways for the Biosynthesis of Novel Antifungals” ACS Cent. Sci. 2020 doi: https://doi.org/10.1021/acscentsci.0c00241

  • Liou, C. S.; Sirk, S. J.; Diaz, C. A. C.; Klein, A. P.; Fischer, C. R.; Higginbottom, S. K.; Erez, A.; Donia, M. S.; Sonnenburg, J. L.; Sattely, E. S. “A Metabolic Pathway for Activation of Dietary Glucosinolates by a Human Gut Symbiont” Cell 2020 doi: https://doi.org/10.1016/j.cell.2020.01.023

  • Jeon, J. E.* & Kim, J.-G.*; Fischer, C. R.; Mehta, N.; Dufour-Schroif, C.; Wemmer, K.; Mudgett, M. B. & Sattely, E. “A Pathogen-Responsive Gene Cluster for Highly Modified Fatty Acids in Tomato” Cell 2020 doi: https://doi.org/10.1016/j.cell.2019.11.037

2019

  • Schultz, B. J.; Kim, S. Y.; Lau, W.; Sattely, E. S. “Total Biosynthesis for Milligram-Scale Production of Etoposide Intermediates in a Plant Chassis” J. Am. Chem. Soc. 2019 doi: https://doi.org/10.1021/jacs.9b10717

  • Holmes, E. C.* & Chen, Y.-C.*; Sattely, E. S. & Mudgett, M. B. “An Engineered Pathway for N-Hydroxy-Pipecolic Acid Synthesis Enhances Systemic Acquired Resistance in Tomato” Sci. Signal. 2019 doi: https://doi.org/10.1126/scisignal.aay3066

  • Hodgson, H.* & De La Peña, R.*; Stephenson, M. J.; Thimmappa, R.; Vincent, J. L.; Sattely, E. S.; Osbourn, A. “Identification of Key Enzymes Responsible for Protolimonoid Biosynthesis in Plants: Opening the Door to Azadirachtin Production” Proc. Natl. Acad. Sci. U. S. A. 2019 doi: https://doi.org/10.1073/pnas.1906083116

  • Voges, M. J. E. E. E.; Bai, Y.; Schulze-Lefert, P.; Sattely, E. S. “Plant-Derived Coumarins Shape the Composition of an Arabidopsis Synthetic Root Microbiome” Proc. Natl. Acad. Sci. U. S. A. 2019 doi: https://doi.org/10.1073/pnas.1820691116

2018

  • Nett, R. S.; Guan, X.; Smith, K.; Faust, A. M.; Sattely, E. S. & Fischer, C. R. “D2O Labeling to Measure Active Biosynthesis of Natural Products in Medicinal Plants” AIChE J. 2018 doi: https://doi.org/10.1002/aic.16413

  • Chen, Y.-C.* & Holmes, E. C.*; Rajniak, J.; Kim, J.-G.; Tang, S.; Fischer, C. R.; Mudgett, M. B. & Sattely, E. S. “N-Hydroxy-Pipecolic Acid Is a Mobile Metabolite That Induces Systemic Disease Resistance in Arabidopsis” Proc. Natl. Acad. Sci. U. S. A. 2018 doi: https://doi.org/10.1073/pnas.1805291115

  • Rajniak, J.; Giehl, R. F. H.; Chang, E.; Murgia, I.; von Wirén, N.; Sattely, E. S. “Biosynthesis of Redox-Active Metabolites in Response to Iron Deficiency in Plants” Nat. Chem. Biol. 2018. doi: https://doi.org/10.1038/s41589-018-0019-2

  • Harvey, C. J. B.* & Tang, M.*; Schlecht, U.; Horecka, J.; Fischer, C. R.; Lin, H.-C.; Li, J.; Naughton, B.; Cherry, J.; Miranda, M.; Li, Y. F.; Chu, A. M.; Hennessy, J. R.; Vandova, G. A.; Inglis, D.; Aiyar, R. S.; Steinmetz, L. M.; Davis, R. W.; Medema, M. H.; Sattely, E.; Khosla, C.; Onge, R. P. S.; Tang, Y.; Hillenmeyer, M. E. “HEx: A Heterologous Expression Platform for the Discovery of Fungal Natural Products” Science Advances 2018 doi: https://doi.org/10.1126/sciadv.aar5459

2017

2016

  • LeFevre, G. H.; Portmann, A. C.; Müller, C. E.; Sattely, E. S.; Luthy, R. G. “Plant Assimilation Kinetics and Metabolism of 2-Mercaptobenzothiazole Tire Rubber Vulcanizers by Arabidopsis” Environ. Sci. Technol. 2016 doi: https://doi.org/10.1021/acs.est.5b04716

  • Müller, C. E.; LeFevre, G. H.; Timofte, A. E.; Hussain, F. A.; Sattely, E. S. & Luthy, R. G. “Competing Mechanisms for Perfluoroalkyl Acid Accumulation in Plants Revealed Using an Arabidopsis Model System” Environ. Toxicol. Chem. 2016 doi: https://doi.org/10.1002/etc.3251

2015

  • Klein, A. P.; Sattely, E. S. “Two Cytochromes P450 Catalyze S-Heterocyclizations in Cabbage Phytoalexin Biosynthesis” Nat. Chem. Biol. 2015 doi: https://doi.org/10.1038/nchembio.1914

  • Rajniak, J.; Barco, B.; Clay, N. K. & Sattely, E. S. “A New Cyanogenic Metabolite in Arabidopsis Required for Inducible Pathogen Defence” Nature 2015 doi: https://doi.org/10.1038/nature14907

  • LeFevre, G. H.; Müller, C. E.; Li, R. J.; Luthy, R. G.; Sattely, E. S. “Rapid Phytotransformation of Benzotriazole Generates Synthetic Tryptophan and Auxin Analogs in Arabidopsis” Environ. Sci. Technol. 2015 doi: https://doi.org/10.1021/acs.est.5b02749

2014

2013

  • Klein, A. P.; Anarat-Cappillino, G.; Sattely, E. S. “Minimum Set of Cytochromes P450 for Reconstituting the Biosynthesis of Camalexin, a Major Arabidopsis Antibiotic” Angew. Chem. Int. Ed Engl. 2013 doi: https://doi.org/10.1002/anie.201307454

  • Chung, Y.-L.; Olsson, J. V.; Li, R. J.; Frank, C. W.; Waymouth, R. M.; Billington, S. L.; Sattely, E. S. “A Renewable Lignin–Lactide Copolymer and Application in Biobased Composites” ACS Sustain. Chem. Eng. 2013 doi: https://doi.org/10.1021/sc4000835

2001-2009

  • Heemstra, J. R., Jr; Walsh, C. T.; Sattely, E. S. “Enzymatic Tailoring of Ornithine in the Biosynthesis of the Rhizobium Cyclic Trihydroxamate Siderophore Vicibactin” J. Am. Chem. Soc. 2009 doi: https://doi.org/10.1021/ja9056008

  • Wuest, W. M.; Sattely, E. S.; Walsh, C. T. “Three Siderophores from One Bacterial Enzymatic Assembly Line” J. Am. Chem. Soc. 2009 doi: https://doi.org/10.1021/ja900815w

  • Sattely, E. S.; Meek, S. J.; Malcolmson, S. J.; Schrock, R. R.; Hoveyda, A. H. “Design and Stereoselective Preparation of a New Class of Chiral Olefin Metathesis Catalysts and Application to Enantioselective Synthesis of Quebrachamine: Catalyst Development Inspired by Natural Product Synthesis” J. Am. Chem. Soc. 2009 doi: https://doi.org/10.1021/ja8084934

  • Malcolmson, S. J.; Meek, S. J.; Sattely, E. S.; Schrock, R. R.; Hoveyda, A. H. “Highly Efficient Molybdenum-Based Catalysts for Enantioselective Alkene Metathesis” Nature 2008 doi: https://doi.org/10.1038/nature07594

  • Sattely, E. S.; Walsh, C. T. “A Latent Oxazoline Electrophile for N-O-C Bond Formation in Pseudomonine Biosynthesis'' J. Am. Chem. Soc. 2008 doi: https://doi.org/10.1021/ja804499r

  • Sattely, E. S.; Fischbach, M. A.; Walsh, C. T. “Total Biosynthesis: In Vitro Reconstitution of Polyketide and Nonribosomal Peptide Pathways” Nat. Prod. Rep. 2008 doi: https://doi.org/10.1039/b801747f

  • Sattely, E. S.; Cortez, G. A.; Moebius, D. C.; Schrock, R. R.; Hoveyda, A. H. “Enantioselective Synthesis of Cyclic Amides and Amines through Mo-Catalyzed Asymmetric Ring-Closing Metathesis” J. Am. Chem. Soc. 2005 doi: https://doi.org/10.1021/ja051330s

  • Dolman, S. J.; Sattely, E. S.; Hoveyda, A. H.; Schrock, R. R. “Efficient Catalytic Enantioselective Synthesis of Unsaturated Amines: Preparation of Small- and Medium-Ring Cyclic Amines through Mo-Catalyzed Asymmetric Ring-Closing Metathesis in the Absence of Solvent” J. Am. Chem. Soc. 2002 doi: https://doi.org/10.1021/ja012534l

  • La, D. S.; Sattely, E. S.; Ford, J. G.; Schrock, R. R.; Hoveyda, A. H. “Catalytic Asymmetric Ring-Opening Metathesis/Cross Metathesis (AROM/CM) Reactions. Mechanism and Application to Enantioselective Synthesis of Functionalized Cyclopentanes” J. Am. Chem. Soc. 2001 doi: https://doi.org/10.1021/ja010684q